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MONOTUB GUIDE

INSTRUCTIONS

Supplies you'll need

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Included in your order:

• Inflatable monotub

• 3 lb grain bag

• 5 lb substrate bag

• Bag clip

• Alcohol wipe

Not Included:

• 10-20 ml Liquid Culture or Spore syringe (see below for purchasing info)

Recommended:

• 70% isopropyl alcohol

• Latex gloves

• Scissors

• Still air box or flow hood

Where to Buy Liquid Culture and Spores:

You can find these items available from many online vendors.

We recommend sporepeople.com. Use code SURF10 for 10% off!

Step 1: Sanitize your work area

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This step is crucial, as any amount of mold or bacteria can contaminate your grow.

1. Turn off any fans, air conditioners, or heaters in the room.

2. Thoroughly wash your hands before putting on gloves.

3. Clean your hands and workstation with 70% isopropyl alcohol.

4. (Recommended) Perform the following step inside a still air box or in front of a flow hood.

Step 2: Inoculate your grain bag

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1. Gently tap your grain bag on your work surface so the grain settles back together.

1. Clean the injection port with the included alcohol wipe.

2. Attach the sterile needle to your syringe. If necessary, sterilize it using a flame and allow it to cool.

3. Carefully insert the needle into the injection port and inject the bag with 10-20ml of liquid culture or spores.

If you're using liquid culture, shake the bag thoroughly to evenly distribute the liquid.

If you're using spores, make sure that all the liquid is in a single spot. Do not shake the bag.

4. Store the grain bag in an area away from direct sunlight, off the ground, with a temperature range of 72-82 degrees Fahrenheit. Note that a temperature in the high 70s to low 80s will result in the fastest colonization time.

Step 3: Break and shake once its 20% colonized

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Once your bag is partially colonized, redistributing the grains will speed up the colonization process.

1. Carefully break apart the colonized sections of the grain bag.

2. Shake the bag until the mycelium is evenly distributed.

3. Place the bag under the same conditions as before. Allow the grains to colonize for an additional 1-2 weeks.

Step 4: Mix your spawn and substrate

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Once your grain bag is fully colonized, it's time to combine it with your substrate.

1. Repeat the steps outlined in "SANITIZE YOUR WORK AREA" to minimize the risk of contamination.

2. Break up your colonized grain spawn by firmly pressing it against a flat surface. Break up all the chunks and mix the bag thoroughly.

4. Cut open the top of both the grain and substrate bags. Transfer your broken-up colonized grain into the substrate bag.

5. Seal the top of the bag with the included clip and shake it to mix the grain and substrate thoroughly.

Step 5: Prep your monotub

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Once your spawn and substrate are mixed, it's time to prepare your monotub.

1. Inflate the monotub using a pump or by blowing into the air valve.

2. Disinfect the monotub and one tub liner with 70% isopropyl alcohol.

3. Position the liner inside the tub. Unclip the substrate bag and carefully pour the mixture into the liner.

4. Distribute the mixture evenly throughout the sides and corners of the liner. Create a flat even surface without compressing the mixture.

5.Insert the blue plugs into all 10 holes of the monotub. Place the monotub off the ground, away from direct sunlight, and at room temperature.

6. Allow the substrate to colonize for 5-7 days before switching to foam plugs

Step 6: Fruiting stage

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Once your grow kit is fully colonized it's time to introduce fruiting conditions.

1. Replace the blue plugs with foam filters. Remove the lid and mist the inside of the monotub.

2. Place your tub in an area with some airflow and indirect sunlight. If using grow lights, opt for 6500k bulbs set to a 12/12 hour cycle timer.

3. Your monotub will begin to pin in about 5-7 days; after that, expect fully grown mushrooms within 3-5 days

Step 7: Harvest mushrooms!

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The best time to harvest your mushrooms is once the cap begins to open and the veil tears.

1. Unseal the bag and carefully slide the grow kit and mushrooms out 

2. Harvest your mushrooms by lightly twisting and pulling them at the base of the stems.

3. (Recommended) Dry your mushrooms using a dehydrator.

4. Store your dried mushrooms in an airtight container with a silica gel pack.

For Multiple Harvests: Repeat steps 4 and 5 for up to two more flushes!

If you have any additional questions feel free to reach out to info@surfinspores.com.

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